Cryo-EM
Uppsala, Sweden · Uppsala University
Quick answer
This core facility in Uppsala, Sweden provides Cryo-EM for Cell biology, Immunology, Oncology, Neuroscience. Reference price: 2,500 to 18,000 SEK per project.
Capabilities
Cryo-EM Uppsala offers the complete workflow for single particle cryo-EM analysis. Sample preparation A Mark IV Vitrobot (Thermo Fisher) is available for sample vitrification. Samples may be screened and analyzed in-house or shipped/transported to another facility for data collection. Sample screening and data collection A 200kV Glacios electron microscope mounted with a Falcon 4i direct electron detector and Selectris energy filter is available for efficient sample screening and/or data collection. The Glacios is equipped with an autoloader that allows loading of up to 12 grids for continuous analysis for up to 3 days. The Falcon 4i direct electron detector is capable of collecting 200-1000 images/hours at high-resolution depending on conditions. Data analysis and image processing Cryo-EM Uppsala houses a local GPU cluster for on-the-fly image processing and sample optimization, and 3D processing and model building using a wide variety of software packages, including Relion and Cryosparc. RATES Booking of the instruments are done using the Cryo-EM Uppsala iLab Booking System. Cancellation should be done at least 24 h before the start of the session. Price: 600 SEK/session (consumables are available at cost through iLab) Glacios Day: 2,500 SEK (6 hrs, 9.00-15.00) Night: 2,500 SEK (18 hrs, 15.00-09.00 the day after) Weekend Data Collection: 7,500 SEK (66 hrs, 15,00 Friday to 09.00 Monday) Non-governmental: 18,000 SEK (24 hrs, 09.00-09.00 the next day)
Location & contact
Reference pricing
| Type | Core facility |
|---|---|
| Technique | Cryo-EM |
| Research areas | Cell biology, Immunology, Oncology, Neuroscience |
| ISO / certificates | N/A |
| City | Uppsala |
| Country | Sweden |
| Provider | Uppsala University |
| Reference price | 2,500 to 18,000 SEK / project |
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